Difference between revisions of "User:Madeline Hartman/Notebook/Dr. Hartings Lab/2013/01/29"

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(Autocreate 2013/01/29 Entry for User:Madeline_Hartman/Notebook/Dr._Hartings_Lab)
 
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==Entry title==
 
==Entry title==
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Gel electrophoresis was run to test PCR
  
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1% Agarose Gel- .25 g agarose
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                                25 mL electrophorsis buffer
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Microwave for 45 seconds to dissolve
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Lane 2- 6 μL marker
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Lane 3- 1 μL dye + 5 μL original DNA
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Lane 4- 1 μL dye + 5μL forward/reverse DNA
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Run at 100 V for 60 minutes
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Results:
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Original- No Band
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Forward/Reverse- Band
  
 
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Revision as of 11:13, 22 September 2013

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Entry title

Gel electrophoresis was run to test PCR

1% Agarose Gel- .25 g agarose

                               25 mL electrophorsis buffer

Microwave for 45 seconds to dissolve

Lane 2- 6 μL marker

Lane 3- 1 μL dye + 5 μL original DNA

Lane 4- 1 μL dye + 5μL forward/reverse DNA

Run at 100 V for 60 minutes

Results:

Original- No Band

Forward/Reverse- Band