User:Madeline Hartman/Notebook/Dr. Hartings Lab/2012/11/06: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==Entry title==
==Entry title==
* Insert content here...
I ran two PCR reactions with the following content:
 
Tube 1-
# 40.6 μL distilled H2O
# 5.0 μL 10x cloned Pfu buffer
# 0.4 μL dNTPs (25 mM each)
# 1.0 μL DNA template (100 ng/μL)
# 1.0 μL Primer 1 (ng/μL)
# 1.0 μL Primer 2 (ng/μL)
# 1.0 μL Pfu Turbo DNA Polymerase (2.5 U/μL)
 
and
 
Tube 2-
# 20.3 μL distilled H2O
# 5.0 μL 10x cloned Pfu buffer
# 0.4 μL dNTPs (25 mM each)
# 1.0 μL DNA template (100 ng/μL)
# 1.0 μL Primer 1 (ng/μL)
# 1.0 μL Pfu Turbo DNA Polymerase (2.5 U/μL)
Tube 3-
# 20.3 μL distilled H2O
# 5.0 μL 10x cloned Pfu buffer
# 0.4 μL dNTPs (25 mM each)
# 1.0 μL DNA template (100 ng/μL)
# 1.0 μL Primer 2 (ng/μL)
# 1.0 μL Pfu Turbo DNA Polymerase (2.5 U/μL)
 
Tubes 2 and 3 were later combined.
 
Primer 1- t109c forward
 
Primer 2- t109c reverse
 
The three tubes were ran for the following cycles:
 
[[Image:MH 20121106Table of cycles.png|300px]]
 
 
After 5 cycles, tubes 2 and 3 were combined, and run the rest of the cycles together.
 
Tubes were left in thermocycler overnight at 4°C





Latest revision as of 22:19, 26 September 2017

Project name Main project page
Next entry

Entry title

I ran two PCR reactions with the following content:

Tube 1-

  1. 40.6 μL distilled H2O
  2. 5.0 μL 10x cloned Pfu buffer
  3. 0.4 μL dNTPs (25 mM each)
  4. 1.0 μL DNA template (100 ng/μL)
  5. 1.0 μL Primer 1 (ng/μL)
  6. 1.0 μL Primer 2 (ng/μL)
  7. 1.0 μL Pfu Turbo DNA Polymerase (2.5 U/μL)

and

Tube 2-

  1. 20.3 μL distilled H2O
  2. 5.0 μL 10x cloned Pfu buffer
  3. 0.4 μL dNTPs (25 mM each)
  4. 1.0 μL DNA template (100 ng/μL)
  5. 1.0 μL Primer 1 (ng/μL)
  6. 1.0 μL Pfu Turbo DNA Polymerase (2.5 U/μL)

Tube 3-

  1. 20.3 μL distilled H2O
  2. 5.0 μL 10x cloned Pfu buffer
  3. 0.4 μL dNTPs (25 mM each)
  4. 1.0 μL DNA template (100 ng/μL)
  5. 1.0 μL Primer 2 (ng/μL)
  6. 1.0 μL Pfu Turbo DNA Polymerase (2.5 U/μL)

Tubes 2 and 3 were later combined.

Primer 1- t109c forward

Primer 2- t109c reverse

The three tubes were ran for the following cycles:


After 5 cycles, tubes 2 and 3 were combined, and run the rest of the cycles together.

Tubes were left in thermocycler overnight at 4°C