Difference between revisions of "User:Madeline Hartman/Notebook/Dr. Hartings Lab/2012/11/06"

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(Autocreate 2012/11/06 Entry for User:Madeline_Hartman/Notebook/Dr._Hartings_Lab)
 
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==Entry title==
 
==Entry title==
* Insert content here...
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I ran two PCR reactions with the following content:
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# 40.6 μL distilled H2O
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# 5.0 μL 10x cloned Pfu buffer
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# 0.4 μL dNTPs (25 mM each)
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# 1.0 μL DNA template (100 ng/μL)
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# 1.0 μL Primer 1 (ng/μL)
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# 1.0 μL Primer 2 (ng/μL)
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# 1.0 μL Pfu Turbo DNA Polymerase (2.5 U/μL)
  
  

Revision as of 08:46, 4 December 2012

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Entry title

I ran two PCR reactions with the following content:

  1. 40.6 μL distilled H2O
  2. 5.0 μL 10x cloned Pfu buffer
  3. 0.4 μL dNTPs (25 mM each)
  4. 1.0 μL DNA template (100 ng/μL)
  5. 1.0 μL Primer 1 (ng/μL)
  6. 1.0 μL Primer 2 (ng/μL)
  7. 1.0 μL Pfu Turbo DNA Polymerase (2.5 U/μL)