Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/10/23"

From OpenWetWare
Jump to: navigation, search
Line 13: Line 13:
 
*Run absorbance measurements
 
*Run absorbance measurements
 
*Use Beer's law to determine concentration
 
*Use Beer's law to determine concentration
===Data===
+
===Data/Calculations===
 
*0.0026g ZnPPIX dissolved in 7mL DMF
 
*0.0026g ZnPPIX dissolved in 7mL DMF
 
*MW ZnPPIX: 626.032g/mol
 
*MW ZnPPIX: 626.032g/mol
Line 19: Line 19:
 
**assuming entire sample came off weigh boat
 
**assuming entire sample came off weigh boat
 
*Beer's Law calculations for absorbance measurements determined the concentration to be ≈0.08mM
 
*Beer's Law calculations for absorbance measurements determined the concentration to be ≈0.08mM
===Notes===
+
**[[Image:2013_1023_ZnPPIX_beers_law_calcs.PNG]]
 +
*Absorbance Spectra and Literature Comparison:
 +
[[Image:2013_1023_ZnPPIX_abs_spectra_(mine).PNG]]<br.>
 +
[[Image:2013_1023_ZnPPIX_abs_spectra.PNG]]<br.>
 +
from "Metal and redox selectivity of protoporphyrin binding to the heme chaperone CcmE"
  
  

Revision as of 06:22, 23 October 2013

Owwnotebook icon.png ZnPPIX Sample Prep <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

October 23, 2013

Happy Mole Day!

Procedures

  • Dissolve filtered ZnPPIX from 10/16/2013 in DMF
  • Prepare several dilutions
  • Run absorbance measurements
  • Use Beer's law to determine concentration

Data/Calculations

  • 0.0026g ZnPPIX dissolved in 7mL DMF
  • MW ZnPPIX: 626.032g/mol
  • (0.0026g)×(1/626.032g/mol)×(1/0.007L)≈0.5933mM
    • assuming entire sample came off weigh boat
  • Beer's Law calculations for absorbance measurements determined the concentration to be ≈0.08mM
    • 2013 1023 ZnPPIX beers law calcs.PNG
  • Absorbance Spectra and Literature Comparison:

2013 1023 ZnPPIX abs spectra (mine).PNG<br.> 2013 1023 ZnPPIX abs spectra.PNG<br.> from "Metal and redox selectivity of protoporphyrin binding to the heme chaperone CcmE"