Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/10/23"

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*Use Beer's law to determine concentration
 
*Use Beer's law to determine concentration
 
===Data===
 
===Data===
*0.0146g ZnPPIX dissolved in 13mL DMF
+
*0.0026g ZnPPIX dissolved in 7mL DMF
 
*MW ZnPPIX: 626.032g/mol
 
*MW ZnPPIX: 626.032g/mol
(0.0026g)×(626.032g/mol)×(1/0.007L)≈0.5933mM<br.>
+
*(0.0026g)×(1/626.032g/mol)×(1/0.007L)≈0.5933mM  
 
+
**assuming entire sample came off weigh boat
 +
*Beer's Law calculations for absorbance measurements determined the concentration to be ≈0.08mM
 
===Notes===
 
===Notes===
  

Revision as of 06:17, 23 October 2013

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October 23, 2013

Happy Mole Day!

Procedures

  • Dissolve filtered ZnPPIX from 10/16/2013 in DMF
  • Prepare several dilutions
  • Run absorbance measurements
  • Use Beer's law to determine concentration

Data

  • 0.0026g ZnPPIX dissolved in 7mL DMF
  • MW ZnPPIX: 626.032g/mol
  • (0.0026g)×(1/626.032g/mol)×(1/0.007L)≈0.5933mM
    • assuming entire sample came off weigh boat
  • Beer's Law calculations for absorbance measurements determined the concentration to be ≈0.08mM

Notes