Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/10/09"

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(Procedures)
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*Procedure adapted from "On the preparation of metalloporphyrins"
 
*Procedure adapted from "On the preparation of metalloporphyrins"
 
**0.5g tetraphenylporphyrin refluxed in 50mL DMF
 
**0.5g tetraphenylporphyrin refluxed in 50mL DMF
**0.403g (stiochiometric amount) zinc acetate added, solution refluxed
+
**0.403g (stiochiometric amount) zinc acetate added, solution refluxed until reaction complete
 +
***completion determination by unchanged peaks in UV-vis measurements
 
**50mL distilled water added, solution chilled and then filtered
 
**50mL distilled water added, solution chilled and then filtered
 
***the filtrate was filtered over again and added to main filtered sample
 
***the filtrate was filtered over again and added to main filtered sample
 +
 
===Data===
 
===Data===
 
Absorbance Measurements
 
Absorbance Measurements

Revision as of 07:25, 16 October 2013

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October 9, 2013

Procedures

  • Synthesize new ZnPPIX with Dr Hartings
  • Procedure adapted from "On the preparation of metalloporphyrins"
    • 0.5g tetraphenylporphyrin refluxed in 50mL DMF
    • 0.403g (stiochiometric amount) zinc acetate added, solution refluxed until reaction complete
      • completion determination by unchanged peaks in UV-vis measurements
    • 50mL distilled water added, solution chilled and then filtered
      • the filtrate was filtered over again and added to main filtered sample

Data

Absorbance Measurements

  • Used to determine reaction completion (based on the change in the peaks)

2012 1009 ZnPPPIX abs.PNG

Notes

Next time:

  • Dissolve filtered ZnPPIX in DMF