Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/10/09"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> UV-vis Measurements & Sample Preparation</span>
 
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==Date==
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==October 2, 2013==
 +
*Absorbance measurements and Beer's law calculations for samples from 10/25/2013
 +
*Sample preparation based on calculated concentrations
 
===Procedures===
 
===Procedures===
 +
  
 
===Data===
 
===Data===
 +
=====Absorbance Measurements from 10/25/2013=====
 +
[[Image:2013_1002_ZnPPIX_from_last_week.PNG]]<br.>
 +
Beer's Law calculations:<br.>
 +
[[Image:2013_1002_ZnPPIX_from_last_week_calcs.PNG]]
  
 +
=====Absorbance Measurements, Today=====
 +
I decided to run two more samples at higher concentrations on the UV-vis and calculate the concentrations from these because I needed higher concentrations in order to make my samples at the concentrations I've been using.
 +
<br.>
 +
[[Image:2013_1002_ZnPPIX_for_calcs.PNG]]<br.>
 +
Beer's Law calculations:<br.>
 +
[[Image:2013_1002_ZnPPIX_calcs.PNG]]
 +
 +
=====Samples Prepared=====
 +
*2.47uM ZnPPIX
 +
*2.47uM ZnPPIX/1.11uM DNA-SH
 +
*2.47uM ZnPPIX/1.11uM DNA (4)
 +
*2.47uM ZnPPIX/1.11uM DNA (8)
 +
*1.934uM ZnPPIX/0.94uM DNA-SH/11.75nM AuNPs
 +
*1.934uM ZnPPIX/0.74uM DNA (4)/11.75nM AuNPs
 +
*1.934uM ZnPPIX/0.74uM DNA (8)/11.75nM AuNPs
 +
*2.47uM ZnPPIX/11.75nM AuNPs
  
 
===Notes===
 
===Notes===
 
+
*Run today's samples for absorbance and fluorescence
 +
*Ask Dr Hartings for more ZnPPIX
  
 
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Revision as of 08:23, 2 October 2013

Owwnotebook icon.png UV-vis Measurements & Sample Preparation <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

October 2, 2013

  • Absorbance measurements and Beer's law calculations for samples from 10/25/2013
  • Sample preparation based on calculated concentrations

Procedures

Data

Absorbance Measurements from 10/25/2013

2013 1002 ZnPPIX from last week.PNG<br.> Beer's Law calculations:<br.> 2013 1002 ZnPPIX from last week calcs.PNG

Absorbance Measurements, Today

I decided to run two more samples at higher concentrations on the UV-vis and calculate the concentrations from these because I needed higher concentrations in order to make my samples at the concentrations I've been using. <br.> 2013 1002 ZnPPIX for calcs.PNG<br.> Beer's Law calculations:<br.> 2013 1002 ZnPPIX calcs.PNG

Samples Prepared
  • 2.47uM ZnPPIX
  • 2.47uM ZnPPIX/1.11uM DNA-SH
  • 2.47uM ZnPPIX/1.11uM DNA (4)
  • 2.47uM ZnPPIX/1.11uM DNA (8)
  • 1.934uM ZnPPIX/0.94uM DNA-SH/11.75nM AuNPs
  • 1.934uM ZnPPIX/0.74uM DNA (4)/11.75nM AuNPs
  • 1.934uM ZnPPIX/0.74uM DNA (8)/11.75nM AuNPs
  • 2.47uM ZnPPIX/11.75nM AuNPs

Notes

  • Run today's samples for absorbance and fluorescence
  • Ask Dr Hartings for more ZnPPIX