Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/09/04"

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Zinc protophorphyrin (IX) sample preparation  
 
Zinc protophorphyrin (IX) sample preparation  
 
===Replicating Fluorescence Samples from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/06/05 6/06/2013]===
 
===Replicating Fluorescence Samples from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/06/05 6/06/2013]===
 +
#2.22uM ZnPPIX
 +
#2.22uM ZnPPIX/1.11uM DNA-SH
 +
#1.934uM ZnPPIX/0.94uM DNA-SH/11.75uM AuNPs
 +
#2.22uM ZnPPIX/11.75uM AuNPs
  
===Replicating Fluorescence from Publication===
+
===Replicating Fluorescence Procedure from Publication===
"Self Assembly of the ZnPPIX/G-Quadruplex Complex" in [http://pubs.acs.org/doi/abs/10.1021/ac300348v Fluorescence Detection of DNA, Adenosine-5′-Triphosphate (ATP), and Telomerase Activity by Zinc(II)-Protoporphyrin IX/G-Quadruplex Labels]
+
"Self Assembly of the ZnPPIX/G-Quadruplex Complex" in [http://pubs.acs.org/doi/abs/10.1021/ac300348v Fluorescence Detection of DNA, Adenosine-5′-Triphosphate (ATP), and Telomerase Activity by Zinc(II)-Protoporphyrin IX/G-Quadruplex Labels]<br.>
 +
#100uL 3uM 4-strand DNA- in place of telomerase solution
 +
#*100uL 50mM KCl and 5uM ZnPPIX (to ensure excess)
 +
#*Allow to react for 10 min
  
 
===Modifying Fluorescence Samples from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/06/05 6/06/2013]===
 
===Modifying Fluorescence Samples from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/06/05 6/06/2013]===

Revision as of 08:50, 4 September 2013

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September 4, 2013

Zinc protophorphyrin (IX) sample preparation

Replicating Fluorescence Samples from 6/06/2013

  1. 2.22uM ZnPPIX
  2. 2.22uM ZnPPIX/1.11uM DNA-SH
  3. 1.934uM ZnPPIX/0.94uM DNA-SH/11.75uM AuNPs
  4. 2.22uM ZnPPIX/11.75uM AuNPs

Replicating Fluorescence Procedure from Publication

"Self Assembly of the ZnPPIX/G-Quadruplex Complex" in Fluorescence Detection of DNA, Adenosine-5′-Triphosphate (ATP), and Telomerase Activity by Zinc(II)-Protoporphyrin IX/G-Quadruplex Labels<br.>

  1. 100uL 3uM 4-strand DNA- in place of telomerase solution
    • 100uL 50mM KCl and 5uM ZnPPIX (to ensure excess)
    • Allow to react for 10 min

Modifying Fluorescence Samples from 6/06/2013