User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/29: Difference between revisions
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[[Image:2013_0729_AuNPs@SiO2.PNG]]<br.> | [[Image:2013_0729_AuNPs@SiO2.PNG]]<br.> | ||
Silica-coated AuNPs made with MHA dissolved in water (heated to 130C in order for MHA to dissolve) vs. MHA dissolved in isopropanol (not heated; MHA soluble in organic solvents). | Silica-coated AuNPs made with MHA dissolved in water (heated to 130C in order for MHA to dissolve) vs. MHA dissolved in isopropanol (not heated; MHA soluble in organic solvents). | ||
====Notes==== | |||
Spectra for silica-coated AuNPs made with MHA dissolved in water is consistent with spectra found in the literature. The noticeable shift towards the right in the spectra for silica-coated AuNPs made with MHA dissolved in isopropanol is indicative of aggregation. Additionally, the AuNPs-MHA in isopropanol solution was a deep purple color, also consistent with aggregation, while the AuNPs-MHA in water was the characteristic pink color of gold nanoparticles in solution. | |||
===For NIST Tomorrow=== | ===For NIST Tomorrow=== | ||
*9.65nm, 23.5nM AuNPs | *9.65nm, 23.5nM AuNPs | ||
*9.65nm, 4.7nM AuNPs with MHA (in water) | *9.65nm, 4.7nM AuNPs with MHA (in water) | ||
*AuNPs@SiO<sub>2</sub> | *AuNPs@SiO<sub>2</sub>- MHA in water | ||
**5uL | **5uL | ||
**4uL | **4uL | ||
Revision as of 10:33, 29 July 2013
 Absorbance Measurements
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July 29, 2013Absorbance Data: AuNPs@SiO2
NotesSpectra for silica-coated AuNPs made with MHA dissolved in water is consistent with spectra found in the literature. The noticeable shift towards the right in the spectra for silica-coated AuNPs made with MHA dissolved in isopropanol is indicative of aggregation. Additionally, the AuNPs-MHA in isopropanol solution was a deep purple color, also consistent with aggregation, while the AuNPs-MHA in water was the characteristic pink color of gold nanoparticles in solution. For NIST Tomorrow
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