User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/25
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July 25, 2013
Procedure Update: MHA Silica-Coating
According to Aggregation and adhesion of gold nanoparticles in phosphate buffer saline, PBS and prolonged sonication actually cause aggregation in gold nanoparticle solutions: "results showed an optimized sonication time of 10 min to reach equilibrium for plain gold nanoparticles in water." Instead of using PBS to dilute all components, filtered MilliQ water will be used to dilute nanoparticles as well as MHA, as the organic solvent in solution prior to the actual silica-coating may have also been affecting observed the gold nanoparticle aggregation. Filtered MilliQ water will be brought up to room temperature as we've noticed cold solutions (whether water or PBS) have seemed to induce aggregation as well.
Fluorescence Data: 4 and 8 Repeat DNA with ThT
Fluorescence measurements taken and calibration curve constructed in order to show the increased fluorescence intensity with increases number of G-quadruplexes formed.<br.> <br.> <br.> <br.> <br.> <br.> <br.>