Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/17"

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(Absorbance Data)
 
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==July 17, 2013==
 
==July 17, 2013==
 
===Absorbance Data: AuNP@SiO<sub>2</sub> Dilutions===
 
===Absorbance Data: AuNP@SiO<sub>2</sub> Dilutions===
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All samples vortexed before running UV-vis measurements to ensure dispersion of nanoparticles in solution.<br.>
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[[Image:2013_0717_5uL_TES-MHA_1.PNG]]  [[Image:2013_0717_5uL_TES-MHA_2.PNG]]<br.>
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<br.>
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[[Image:2013_0717_10uL_TES-MHA.PNG]][[Image:2013_0717_15uL_TES-MHA.PNG]]<br.>
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<br.>
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[[Image:2013_0717_20uL_TES-MHA.PNG]]  [[Image:2013_0717_dilution_comparison.PNG]]<br.>
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<br.>
  
 
===DNA and AuNP Attachment Procedure===
 
===DNA and AuNP Attachment Procedure===
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Replicate procedure from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/07/15 07/15/2013] with 268mM sodium citrate buffer.
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====Absorbance Data====
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[[Image:2013_0717_AuNP-DNA_&_sups.PNG]]<br.>
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Corrected, with HEPES buffer used as reference solution.<br.>
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Calculations regarding the percentages of AuNPs and DNA in the solution and each supernatant will be done.<br.>
  
===Bring to NIST Tomorrow===
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===For NIST Tomorrow===
*AuNP@SiO<sub>2</sub> samples (16)
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*AuNP@SiO<sub>2</sub> samples in PBS and in water (26)
 
*AuNP samples (3)
 
*AuNP samples (3)
 
*Notebook
 
*Notebook
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*Read over [http://www.nist.gov/customcf/get_pdf.cfm?pub_id=854083 Measuring the Size of Nanoparticles Using Transmission Electron Microscopy (TEM)]
  
  

Latest revision as of 11:04, 17 July 2013

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July 17, 2013

Absorbance Data: AuNP@SiO2 Dilutions

All samples vortexed before running UV-vis measurements to ensure dispersion of nanoparticles in solution.<br.> 2013 0717 5uL TES-MHA 1.PNG 2013 0717 5uL TES-MHA 2.PNG<br.> <br.> 2013 0717 10uL TES-MHA.PNG2013 0717 15uL TES-MHA.PNG<br.> <br.> 2013 0717 20uL TES-MHA.PNG 2013 0717 dilution comparison.PNG<br.> <br.>

DNA and AuNP Attachment Procedure

Replicate procedure from 07/15/2013 with 268mM sodium citrate buffer.

Absorbance Data

2013 0717 AuNP-DNA & sups.PNG<br.> Corrected, with HEPES buffer used as reference solution.<br.> Calculations regarding the percentages of AuNPs and DNA in the solution and each supernatant will be done.<br.>

For NIST Tomorrow