User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/12: Difference between revisions
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#*Stir solutions for one hour to allow reaction to proceed | #*Stir solutions for one hour to allow reaction to proceed | ||
#*Centrifuge solutions at 4000 rpm for one hour | #*Centrifuge solutions at 4000 rpm for one hour | ||
#*Re-suspend particles in PBS | #*Re-suspend particles in 140uL PBS (per solution) | ||
Revision as of 05:41, 17 July 2013
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July 12, 2013Notes from Meeting
DNA and AuNP Attachment ProcedureCalculations
NotesProcedure stopped because the nanoparticles could not be extracted by centrifugation. Absorbance data to be taken tomorrow. Procedure Update: Silica-coating AuNPs by MHAWe decided to follow the MHA procedure because MHA reduces the number of gold cores per silica-coated particles, and we would ideally have each individual gold nanoparticle covered by a layer of silica. Using the MHA versus not using it has also been very effective in the trials conducted so far, and we think that using MHA will allow us to reach our desired optimal shell thickness of approximately 35nm.<br.>
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