User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/12: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Meeting, DNA/AuNP Attachment</span>
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Revision as of 08:39, 12 July 2013

Meeting, DNA/AuNP Attachment <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

July 12, 2013

Notes from Meeting

  • Adjust range and use SymphoTime to better fit FCS graphs in Igor
  • Use error bars on calibration graphs (for Igor <N> values)
  • Focus on attaching AuNPs to DNA & hybridizing MB to run on FCS
  • Use a range of volumes of TES in MHA silica-coating procedure to change silica-coating thickness
    • Take absorbance data to see differences between solution, then use TEM imaging to verify presence of silica-coating

DNA and AuNP Attachment Procedure

Calculations

Done by Allison Alix.<br.> Combine 231.3uL 1.03uM DNA, 231.3uL 29.67nM 14.7nm AuNPs, and 37.3uL sodium citrate buffer prepared 07/11/2013 <br.>



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