Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/11"

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**600pM DNA/100pM MB
 
**600pM DNA/100pM MB
 
**Run three times, 500s each
 
**Run three times, 500s each
**All samples heated to ~70C for 25min, cooled for at least 20min, and vortexed before running
+
**All samples heated to ~70°C for 25min, cooled for at least 20min, and vortexed before running
 
===FCS Measurements===
 
===FCS Measurements===
 
====From samples run 07/10/2013====
 
====From samples run 07/10/2013====
 
+
[[Image:2013_0710_OD_both_compliations.PNG]]
 
====From samples run 07/11/2013====
 
====From samples run 07/11/2013====
 
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[[Image:2013_0711_PBS_compliation.PNG]]<br.>
 +
<br.>
 +
[[Image:2013_0711 all DNA-MB.PNG]]<br.>
  
 
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Revision as of 10:50, 15 July 2013

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July 11, 2013

FCS Sample Prep

  • Green fluorescent beads
    • 10000x diluted
    • Run three times, 90s each
    • Used to properly align laser
    • Vortexed before running
  • DNA/MB
    • 600pM DNA/500pM MB
    • 600pM DNA/400pM MB
    • 600pM DNA/300pM MB
    • 600pM DNA/200pM MB
    • 600pM DNA/100pM MB
    • Run three times, 500s each
    • All samples heated to ~70°C for 25min, cooled for at least 20min, and vortexed before running

FCS Measurements

From samples run 07/10/2013

2013 0710 OD both compliations.PNG

From samples run 07/11/2013

2013 0711 PBS compliation.PNG<br.> <br.> 2013 0711 all DNA-MB.PNG<br.>