Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/11"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> FCS Measurements</span>
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
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==Entry title==
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==July 11, 2013==
* Insert content here...
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===FCS Sample Prep===
 
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*Green fluorescent beads
 +
**10000x diluted
 +
**Run three times, 90s each
 +
**Used to properly align laser
 +
**Vortexed before running
 +
*DNA/MB
 +
**600pM DNA/500pM MB
 +
**600pM DNA/400pM MB
 +
**600pM DNA/300pM MB
 +
**600pM DNA/200pM MB
 +
**600pM DNA/100pM MB
 +
**Run three times, 500s each
 +
**All samples heated to ~70°C for 25min, cooled for at least 20min, and vortexed before running
 +
===FCS Measurements===
 +
====From samples run 07/10/2013====
 +
[[Image:2013_0710_OD_both_compliations.PNG]]
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====From samples run 07/11/2013====
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[[Image:2013_0711_PBS_compliation.PNG]]<br.>
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<br.>
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[[Image:2013_0711 all DNA-MB.PNG]]<br.>
  
 
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Latest revision as of 11:50, 15 July 2013

Owwnotebook icon.png FCS Measurements <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>      </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

July 11, 2013

FCS Sample Prep

  • Green fluorescent beads
    • 10000x diluted
    • Run three times, 90s each
    • Used to properly align laser
    • Vortexed before running
  • DNA/MB
    • 600pM DNA/500pM MB
    • 600pM DNA/400pM MB
    • 600pM DNA/300pM MB
    • 600pM DNA/200pM MB
    • 600pM DNA/100pM MB
    • Run three times, 500s each
    • All samples heated to ~70°C for 25min, cooled for at least 20min, and vortexed before running

FCS Measurements

From samples run 07/10/2013

2013 0710 OD both compliations.PNG

From samples run 07/11/2013

2013 0711 PBS compliation.PNG<br.> <br.> 2013 0711 all DNA-MB.PNG<br.>