User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/11: Difference between revisions

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(Autocreate 2013/07/11 Entry for User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence)
 
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==Entry title==
==July 11, 2013==
* Insert content here...
===FCS Sample Prep===
*Green fluorescent beads
**10000x diluted
**Run three times, 90s each
**Used to properly align laser
*DNA/MB
**600pM DNA/500pM MB
**600pM DNA/400pM MB
**600pM DNA/300pM MB
**600pM DNA/200pM MB
**600pM DNA/100pM MB
**Run three times, 500s each
===FCS Measurements===
====From samples run 07/10/2013====
 
====From samples run 07/11/2013====





Revision as of 06:20, 11 July 2013

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July 11, 2013

FCS Sample Prep

  • Green fluorescent beads
    • 10000x diluted
    • Run three times, 90s each
    • Used to properly align laser
  • DNA/MB
    • 600pM DNA/500pM MB
    • 600pM DNA/400pM MB
    • 600pM DNA/300pM MB
    • 600pM DNA/200pM MB
    • 600pM DNA/100pM MB
    • Run three times, 500s each

FCS Measurements

From samples run 07/10/2013

From samples run 07/11/2013