Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/11"

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(Autocreate 2013/07/11 Entry for User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence)
 
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==Entry title==
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==July 11, 2013==
* Insert content here...
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===FCS Sample Prep===
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*Green fluorescent beads
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**10000x diluted
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**Run three times, 90s each
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**Used to properly align laser
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*DNA/MB
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**600pM DNA/500pM MB
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**600pM DNA/400pM MB
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**600pM DNA/300pM MB
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**600pM DNA/200pM MB
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**600pM DNA/100pM MB
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**Run three times, 500s each
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===FCS Measurements===
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====From samples run 07/10/2013====
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====From samples run 07/11/2013====
  
  

Revision as of 06:20, 11 July 2013

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July 11, 2013

FCS Sample Prep

  • Green fluorescent beads
    • 10000x diluted
    • Run three times, 90s each
    • Used to properly align laser
  • DNA/MB
    • 600pM DNA/500pM MB
    • 600pM DNA/400pM MB
    • 600pM DNA/300pM MB
    • 600pM DNA/200pM MB
    • 600pM DNA/100pM MB
    • Run three times, 500s each

FCS Measurements

From samples run 07/10/2013

From samples run 07/11/2013