Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/10"

From OpenWetWare
Jump to: navigation, search
(July 10, 2013)
(3 intermediate revisions by the same user not shown)
Line 7: Line 7:
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
==July 10, 2013==
 
==July 10, 2013==
 +
===DNA/AuNP Attachement===
 +
*Extraction procedure from Allison Alix on [http://openwetware.org/wiki/User:Allison_K._Alix/Notebook/Thesis_Research/2013/05/31 05/31/2013]
 
===FCS Sample Prep===
 
===FCS Sample Prep===
 
*Green fluorescent beads
 
*Green fluorescent beads
Line 20: Line 22:
 
**50pM
 
**50pM
 
**Run three times, 500s per sample
 
**Run three times, 500s per sample
===FCS Measurements===
 
  
  

Revision as of 05:21, 11 July 2013

Owwnotebook icon.png FCS Measurements <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

July 10, 2013

DNA/AuNP Attachement

  • Extraction procedure from Allison Alix on 05/31/2013

FCS Sample Prep

  • Green fluorescent beads
    • 10000x diluted
    • Run three times, 90s per sample
  • Oligo D
    • 200pM
    • 175pM
    • 150pM
    • 125pM
    • 100pM
    • 75pM
    • 50pM
    • Run three times, 500s per sample