Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/05"

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(July 5, 2013)
(July 5, 2013)
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===FCS Sample Prep===
 
===FCS Sample Prep===
 
*Green fluorescent beads
 
*Green fluorescent beads
**10000x diluted
+
**10000x diluted, run 3x for 90s
 +
*PBS
 +
**To check for background, run 3x for 90s
 
*DNA/MB
 
*DNA/MB
**125/100pM
+
**125/100pM, run 3x for 500s
**100/80pM
+
**100/80pM, run 3x for 500s
**75/60pM
+
**75/60pM, run 3x for 500s
**50/40pM
+
**50/40pM, run 3x for 500s
**25/20pM
+
**25/20pM, run 3x for 500s
 
===FCS Data===
 
===FCS Data===
 
====Notes====
 
====Notes====

Revision as of 05:48, 5 July 2013

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July 5, 2013

FCS Sample Prep

  • Green fluorescent beads
    • 10000x diluted, run 3x for 90s
  • PBS
    • To check for background, run 3x for 90s
  • DNA/MB
    • 125/100pM, run 3x for 500s
    • 100/80pM, run 3x for 500s
    • 75/60pM, run 3x for 500s
    • 50/40pM, run 3x for 500s
    • 25/20pM, run 3x for 500s

FCS Data

Notes