Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/07/01"

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(July 1, 2013)
(July 1, 2013)
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===FCS Sample Preparation===
 
===FCS Sample Preparation===
 
*Green fluorescent beads
 
*Green fluorescent beads
**1000x diluted
+
**1000x diluted: from 10x dilution
**10,000x diluted
+
**10,000x diluted: from 10x dilution
*Oligo D
+
*Oligo D: 20,000pM → 1000pM → 150pM
**150pM
+
**150pM: from 1000pM dilution
**125pM
+
**125pM: from 150pM dilution
**100pM
+
**100pM: from 150pM dilution
**80pM
+
**80pM: from 150pM dilution
**60pM
+
**60pM: from 150pM dilution
**50pM
+
**50pM: from 150pM dilution
**40pM
+
**40pM: from 150pM dilution
**30pM
+
**30pM: from 150pM dilution
**20pM
+
**20pM: from 150pM dilution
**10pM
+
**10pM: from 150pM dilution
 
*MB/DNA
 
*MB/DNA
**1nM/800pM
+
**1nM/800pM: from 2nM/1.6nM dilutions
**900pM/720pM
+
**900pM/720pM: from 50nM dilutions
**800pM/640pM
+
**800pM/640pM: from 50nM dilutions
**700pM/560pM
+
**700pM/560pM: from 2nM/1.6nM dilutions
**600pM/480pM
+
**600pM/480pM: from 2nM/1.6nM dilutions
**500pM/400pM
+
**500pM/400pM: from 2nM/1.6nM dilutions
**400pM/320pM
+
**400pM/320pM: from 2nM/1.6nM dilutions
**300pM/240pM
+
**300pM/240pM: from 2nM/1.6nM dilutions
**200pM/160pM
+
**200pM/160pM: from 2nM/1.6nM dilutions
**100pM/80pM
+
**100pM/80pM: from 2nM/1.6nM dilutions
**75pM/60pM
+
**75pM/60pM: from 2nM/1.6nM dilutions
**50pM/40pM
+
**50pM/40pM: from 2nM/1.6nM dilutions
**25pM/12.5pM
+
**25pM/12.5pM: from 2nM/1.6nM dilutions
 
===FCS Data===
 
===FCS Data===
  

Revision as of 07:17, 1 July 2013

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July 1, 2013

FCS Sample Preparation

  • Green fluorescent beads
    • 1000x diluted: from 10x dilution
    • 10,000x diluted: from 10x dilution
  • Oligo D: 20,000pM → 1000pM → 150pM
    • 150pM: from 1000pM dilution
    • 125pM: from 150pM dilution
    • 100pM: from 150pM dilution
    • 80pM: from 150pM dilution
    • 60pM: from 150pM dilution
    • 50pM: from 150pM dilution
    • 40pM: from 150pM dilution
    • 30pM: from 150pM dilution
    • 20pM: from 150pM dilution
    • 10pM: from 150pM dilution
  • MB/DNA
    • 1nM/800pM: from 2nM/1.6nM dilutions
    • 900pM/720pM: from 50nM dilutions
    • 800pM/640pM: from 50nM dilutions
    • 700pM/560pM: from 2nM/1.6nM dilutions
    • 600pM/480pM: from 2nM/1.6nM dilutions
    • 500pM/400pM: from 2nM/1.6nM dilutions
    • 400pM/320pM: from 2nM/1.6nM dilutions
    • 300pM/240pM: from 2nM/1.6nM dilutions
    • 200pM/160pM: from 2nM/1.6nM dilutions
    • 100pM/80pM: from 2nM/1.6nM dilutions
    • 75pM/60pM: from 2nM/1.6nM dilutions
    • 50pM/40pM: from 2nM/1.6nM dilutions
    • 25pM/12.5pM: from 2nM/1.6nM dilutions

FCS Data