Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/27"

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(June 27, 2013)
(FCS Measurements)
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===FCS Measurements===
 
===FCS Measurements===
 
*Samples were prepared again for the Oligo D and DNA/MB
 
*Samples were prepared again for the Oligo D and DNA/MB
**Oligo D concentrations: 20, 40, 60, 80, 100p<
+
 
**
 
 
   
 
   
  

Revision as of 12:31, 28 June 2013

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June 27, 2013

DNA/ThT Samples for Calibration Graph

  • Remade samples at 500, 600, 700, 800, 900nM
  • Fluorescence measurements taken

Results

Fluor data DNA ThT compliation.png<br.> <br.> Fluor data DNA ThT compliation calibration.png<br.> <br.>

Inconsistent Spectra

Fluor data 600nM DNA comparison.png<br.> <br.> Fluor data 700nM DNA comparison.png<br.> <br.> Fluor data 800nM DNA comparison.png<br.> <br.>

Notes

We still cannot produce consistent spectra for concentrations above 500nM. This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency.

FCS Measurements

  • Samples were prepared again for the Oligo D and DNA/MB