Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/27"

From OpenWetWare
Jump to: navigation, search
(Notes)
(June 27, 2013)
Line 22: Line 22:
 
[[Image:Fluor_data_800nM_DNA_comparison.png]]<br.>
 
[[Image:Fluor_data_800nM_DNA_comparison.png]]<br.>
 
<br.>
 
<br.>
===Notes===
+
====Notes====
 
We still cannot produce consistent spectra for concentrations above 500nM.  This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency.
 
We still cannot produce consistent spectra for concentrations above 500nM.  This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency.
 
+
===FCS Measurements===
 +
*Samples were prepared again for the Oligo D and DNA/MB
 +
**Oligo D concentrations: 20, 40, 60, 80, 100p<
 +
**
 
   
 
   
  

Revision as of 10:21, 27 June 2013

Owwnotebook icon.png Fluorescence and FCS Data <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

June 27, 2013

DNA/ThT Samples for Calibration Graph

  • Remade samples at 500, 600, 700, 800, 900nM
  • Fluorescence measurements taken

Results

Fluor data DNA ThT compliation.png<br.> <br.> Fluor data DNA ThT compliation calibration.png<br.> <br.>

Inconsistent Spectra

Fluor data 600nM DNA comparison.png<br.> <br.> Fluor data 700nM DNA comparison.png<br.> <br.> Fluor data 800nM DNA comparison.png<br.> <br.>

Notes

We still cannot produce consistent spectra for concentrations above 500nM. This may be due to a limit of bonding between the DNA and ThT: only a certain about of ThT molecules can bind to a given number of DNA molecules and that number has been reached, making higher concentrations irrelevant, which would explain the inconsistency.

FCS Measurements

  • Samples were prepared again for the Oligo D and DNA/MB
    • Oligo D concentrations: 20, 40, 60, 80, 100p<