Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/27"

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==Entry title==
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==June 27, 2013==
* Insert content here...
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===DNA/ThT Samples for Calibration Graph===
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*Remade samples at 500, 600, 700, 800, 900nM
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*Fluorescence measurements taken
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====Results====
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[[Image:Fluor_data_DNA_ThT_compliation.png]]<br.>
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<br.>
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[[Image:Fluor_data_DNA_ThT_compliation_calibration.png]]<br.>
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<br.>
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=====Inconsistent Spectra=====
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[[Image:Fluor_data_600nM_DNA_comparison.png]]<br.>
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<br.>
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[[Image:Fluor_data_700nM_DNA_comparison.png]]<br.>
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<br.>
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[[Image:Fluor_data_800nM_DNA_comparison.png]]<br.>
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<br.>
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===Notes===
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We still cannot produce consistent spectra for concentrations about 500nM.  This may be due to a limit of bonding: only a certain about of ThT molecules can bind to a give number of DNA molecules and that number has been reached, making higher concentrations irrelevant.
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Revision as of 08:00, 27 June 2013

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June 27, 2013

DNA/ThT Samples for Calibration Graph

  • Remade samples at 500, 600, 700, 800, 900nM
  • Fluorescence measurements taken

Results

Fluor data DNA ThT compliation.png<br.> <br.> Fluor data DNA ThT compliation calibration.png<br.> <br.>

Inconsistent Spectra

Fluor data 600nM DNA comparison.png<br.> <br.> Fluor data 700nM DNA comparison.png<br.> <br.> Fluor data 800nM DNA comparison.png<br.> <br.>

Notes

We still cannot produce consistent spectra for concentrations about 500nM. This may be due to a limit of bonding: only a certain about of ThT molecules can bind to a give number of DNA molecules and that number has been reached, making higher concentrations irrelevant.