Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/21"

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(June 21, 2013)
(June 21, 2013)
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==June 21, 2013==
 
==June 21, 2013==
 
===Notes from Meeting===
 
===Notes from Meeting===
*
+
*Check pH of sodium citrate buffer (color change?)
 +
**Read literature for salt effects on AuNPs
 +
*Make lower concentration dilutions of DNA/ThT solutions by serial dilutions
 +
**Plot the ratio rather than the DNA concentration
 +
*Use the same wavelength for the calibration curve; take zero point (ThT) out of the calibration curve
 +
*Tuesday: run MB samples on the FCS with Dr Miller
 +
*Dilute 10x AuNPs@SiO<sub>2</sub>
 +
**Read literature for alternative detection methods
 +
*Endnote
 
===Absorbance Data===
 
===Absorbance Data===
  

Revision as of 08:24, 21 June 2013

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June 21, 2013

Notes from Meeting

  • Check pH of sodium citrate buffer (color change?)
    • Read literature for salt effects on AuNPs
  • Make lower concentration dilutions of DNA/ThT solutions by serial dilutions
    • Plot the ratio rather than the DNA concentration
  • Use the same wavelength for the calibration curve; take zero point (ThT) out of the calibration curve
  • Tuesday: run MB samples on the FCS with Dr Miller
  • Dilute 10x AuNPs@SiO2
    • Read literature for alternative detection methods
  • Endnote

Absorbance Data