User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/20: Difference between revisions
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*Run absorbance and fluorescence | *Run absorbance and fluorescence | ||
*Create calibration graph | *Create calibration graph | ||
====Fluorescence Data==== | |||
[[Image:Fluor_data_varying_DNA%2C_2.5_ThT.png]] | |||
====Calibration Graph==== | |||
[[Image:Fluor_data_varying_DNA,_2.5_ThT_calibration_graph.png]] | |||
===Silica-Coating Procedure=== | ===Silica-Coating Procedure=== | ||
*Silica-coating procedure from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/05/24 05/24/2013] performed | *Silica-coating procedure from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/05/24 05/24/2013] performed | ||
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**1mL AuNPs/40uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol | **1mL AuNPs/40uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol | ||
**1mL AuNPs and MHA solution/5uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol | **1mL AuNPs and MHA solution/5uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol | ||
* | *Each of the three solutions were centrifuged at 4000rpm for 1 hour | ||
*Each of the three solutions were re-dispersed in 140uL PBS buffer | |||
====Absorbance Spectra==== | |||
[[Image:Abs_data_silica-coating_0620.png]]<br.> | |||
Absorbance was not observed as expected. Samples may be diluted further in an attempt to eliminate noise from spectra. | |||
Revision as of 12:32, 20 June 2013
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June 20, 2013Sample Prep: New DNA/ThT Solutions
Fluorescence DataCalibration GraphSilica-Coating Procedure
Absorbance Spectra<br.> Absorbance was not observed as expected. Samples may be diluted further in an attempt to eliminate noise from spectra.
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