Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/20"

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(Entry title)
(June 20, 2013)
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*Run absorbance and fluorescence
 
*Run absorbance and fluorescence
 
*Create calibration graph
 
*Create calibration graph
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====Fluorescence Data====
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[[Image:Fluor_data_varying_DNA%2C_2.5_ThT.png]]
 +
====Calibration Graph====
 +
[[Image:Fluor_data_varying_DNA,_2.5_ThT_calibration_graph.png]]
 
===Silica-Coating Procedure===
 
===Silica-Coating Procedure===
 
*Silica-coating procedure from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/05/24 05/24/2013] performed
 
*Silica-coating procedure from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/05/24 05/24/2013] performed
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**1mL AuNPs/40uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol
 
**1mL AuNPs/40uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol
 
**1mL AuNPs and MHA solution/5uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol
 
**1mL AuNPs and MHA solution/5uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol
*Take absorbance spectra of original AuNPs and three silica-coated AuNP solutions
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*Each of the three solutions were centrifuged at 4000rpm for 1 hour
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*Each of the three solutions were re-dispersed in 140uL PBS buffer
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====Absorbance Spectra====
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[[Image:Abs_data_silica-coating_0620.png]]<br.>
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Absorbance was not observed as expected. Samples may be diluted further in an attempt to eliminate noise from spectra.
  
  

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June 20, 2013

Sample Prep: New DNA/ThT Solutions

  • Prepare new 1000nM, 1200nM, 1455nM, 2910nM, and 4000nM DNA with 2.5uM ThT samples
  • Run absorbance and fluorescence
  • Create calibration graph

Fluorescence Data

Fluor data varying DNA, 2.5 ThT.png

Calibration Graph

Fluor data varying DNA, 2.5 ThT calibration graph.png

Silica-Coating Procedure

  • Silica-coating procedure from 05/24/2013 performed
    • 1mL AuNPs/20uL TES/0.125mL NH4OH/5mL isopropanol
    • 1mL AuNPs/40uL TES/0.125mL NH4OH/5mL isopropanol
    • 1mL AuNPs and MHA solution/5uL TES/0.125mL NH4OH/5mL isopropanol
  • Each of the three solutions were centrifuged at 4000rpm for 1 hour
  • Each of the three solutions were re-dispersed in 140uL PBS buffer

Absorbance Spectra

Abs data silica-coating 0620.png<br.> Absorbance was not observed as expected. Samples may be diluted further in an attempt to eliminate noise from spectra.