User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/20: Difference between revisions
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== | ==June 20, 2013== | ||
* | ===Sample Prep: New DNA/ThT Solutions=== | ||
*Prepare new 1000nM, 1200nM, 1455nM, 2910nM, and 4000nM DNA with 2.5uM ThT samples | |||
*Run absorbance and fluorescence | |||
*Create calibration graph | |||
===Silica-Coating Procedure=== | |||
*Silica-coating procedure from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/05/24 05/24/2013] performed | |||
**1mL AuNPs/20uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol | |||
**1mL AuNPs/40uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol | |||
**1mL AuNPs and MHA solution/5uL TES/0.125mL NH<sub>4</sub>OH/5mL isopropanol | |||
*Take absorbance spectra of original AuNPs and three silica-coated AuNP solutions | |||
Revision as of 08:21, 20 June 2013
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June 20, 2013Sample Prep: New DNA/ThT Solutions
Silica-Coating Procedure
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