Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/20"

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Latest revision as of 21:46, 26 September 2017

Owwnotebook icon.png Absorbance Spectra and Calibration Graph Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

June 20, 2013

Sample Prep: New DNA/ThT Solutions

  • Prepare new 1000nM, 1200nM, 1455nM, 2910nM, and 4000nM DNA with 2.5uM ThT samples
  • Run absorbance and fluorescence
  • Create calibration graph

Fluorescence Data

Fluor data varying DNA, 2.5 ThT.png

Calibration Graph

Fluor data varying DNA, 2.5 ThT calibration graph.png

Silica-Coating Procedure

  • Silica-coating procedure from 05/24/2013 performed
    • 1mL AuNPs/20uL TES/0.125mL NH4OH/5mL isopropanol
    • 1mL AuNPs/40uL TES/0.125mL NH4OH/5mL isopropanol
    • 1mL AuNPs and MHA solution/5uL TES/0.125mL NH4OH/5mL isopropanol
  • Each of the three solutions were centrifuged at 4000rpm for 1 hour
  • Each of the three solutions were re-dispersed in 140uL PBS buffer

Absorbance Spectra

Abs data silica-coating 0620.png<br.> Absorbance was not observed as expected. Samples may be diluted further in an attempt to eliminate noise from spectra.