Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/04/23"

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(April 23, 2013)
(April 23, 2013)
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=====Absorbance and Florescence Data of thiol DNA and ThT=====
 
=====Absorbance and Florescence Data of thiol DNA and ThT=====
 
[[Image:4.23.13_absorbance_DNA_and_Tht.png]]
 
[[Image:4.23.13_absorbance_DNA_and_Tht.png]]
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<br.>
 
<br.>
 
<br.>
 
[[Image:4.23.13_florescence_DNA_and_ThT.png]]
 
[[Image:4.23.13_florescence_DNA_and_ThT.png]]
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=====Preparation of Gel and Samples=====
 
=====Preparation of Gel and Samples=====
 
Procedure from Allison Alix on 2013/03/29 [http://openwetware.org/wiki/User:Allison_K._Alix/Notebook/Thesis_Research/2013/03/29]
 
Procedure from Allison Alix on 2013/03/29 [http://openwetware.org/wiki/User:Allison_K._Alix/Notebook/Thesis_Research/2013/03/29]
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 +
=====Attached AuNP to thiol DNA with ThT=====
 +
Combine 250μL DNA/ThT solution with 250μL 4% TEA 100mM DNA<br.>
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Allow solution to react for 10 minutes<br.>
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Follow same extraction procedure from March 19, 2013 [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence/2013/03/19]
  
  

Revision as of 08:49, 24 April 2013

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April 23, 2013

Absorbance and Florescence Data of thiol DNA and ThT

4.23.13 absorbance DNA and Tht.png <br.> <br.> 4.23.13 florescence DNA and ThT.png <br.>

Preparation of Gel and Samples

Procedure from Allison Alix on 2013/03/29 [1]

Attached AuNP to thiol DNA with ThT

Combine 250μL DNA/ThT solution with 250μL 4% TEA 100mM DNA<br.> Allow solution to react for 10 minutes<br.> Follow same extraction procedure from March 19, 2013 [2]