User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/04/16: Difference between revisions
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=====Gel Electrophoresis===== | =====Gel Electrophoresis===== | ||
Procedure from Allison Alix | Procedure from Allison Alix [[http://openwetware.org/wiki/User:Allison_K._Alix/Notebook/Thesis_Research/2013/04/16| 2013/04/16]] | ||
=====Calculations for thiol DNA and ThT solution===== | =====Calculations for thiol DNA and ThT solution===== |
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April 16, 2013Reconstituting DNA and Supernatants10μL of water used to reconstitute each sample of DNA and each of the four supernatants Gel ElectrophoresisProcedure from Allison Alix [2013/04/16] Calculations for thiol DNA and ThT solution
DNA required starting concentration: 5.82μM <br.> (53.1μM)V=(5.82μM)(500μL)<br.> V= 54.8μL 53.1μM thiol DNA in 445.2μL buffer<br.>
<br.> Combine 250μL 5.82μM thiol DNA and 250μL 5μM ThT
<br.> Procedure continued on April 23, 2013<br.> <br.> |