Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/04/16"

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(April 16, 2013)
(Calculations for thiol DNA and ThT solution)
Line 16: Line 16:
 
*AuNP starting concentration: 19.4nM
 
*AuNP starting concentration: 19.4nM
 
*DNA starting concentration: 53.1μM
 
*DNA starting concentration: 53.1μM
DNA required starting concentration: 5.82μM
+
DNA required starting concentration: 5.82μM <br.>
(53.1μM)V=(5.82μM)(500μL)
+
(53.1μM)V=(5.82μM)(500μL)<br.>
V= 54.8μL 53.1μM thiol DNA in 445.2μL buffer
+
V= 54.8μL 53.1μM thiol DNA in 445.2μL buffer<br.>
*thiol DNA concentration required when combined with AuNP: 75×(19.4nM)=1455nM
+
*thiol DNA concentration required when combined with AuNP: 75×(19.4nM)=1455nM<br.>
 
+
<br.>
 
Combine 250μL 5.82μM thiol DNA and 250μL 5μM ThT
 
Combine 250μL 5.82μM thiol DNA and 250μL 5μM ThT
 
*DNA concentration: 2.91μM
 
*DNA concentration: 2.91μM
 
*ThT concentration: 2.5μM
 
*ThT concentration: 2.5μM
 
+
<br.>
Procedure continued on April 23, 2013
+
Procedure continued on April 23, 2013<br.>
 
+
<br.>
  
 
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Revision as of 07:56, 24 April 2013

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April 16, 2013

Reconstituting DNA and Supernatants

10μL of water used to reconstitute each sample of DNA and each of the four supernatants

Gel Electrophoresis

Procedure from Allison Alix 2013/04/16 [[1]]

Calculations for thiol DNA and ThT solution
  • AuNP starting concentration: 19.4nM
  • DNA starting concentration: 53.1μM

DNA required starting concentration: 5.82μM <br.> (53.1μM)V=(5.82μM)(500μL)<br.> V= 54.8μL 53.1μM thiol DNA in 445.2μL buffer<br.>

  • thiol DNA concentration required when combined with AuNP: 75×(19.4nM)=1455nM<br.>

<br.> Combine 250μL 5.82μM thiol DNA and 250μL 5μM ThT

  • DNA concentration: 2.91μM
  • ThT concentration: 2.5μM

<br.> Procedure continued on April 23, 2013<br.> <br.>