Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/04/16"

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(April 16, 2013)
(April 16, 2013)
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==April 16, 2013==
 
==April 16, 2013==
 
=====Reconstituting DNA and Supernatants=====
 
=====Reconstituting DNA and Supernatants=====
 +
10μL of water used to reconstitute each sample of DNA and each of the four supernatants
  
 +
=====Gel Electrophoresis=====
 +
Procedure from Allison Alix 2013/04/16 [[http://openwetware.org/wiki/User:Allison_K._Alix/Notebook/Thesis_Research/2013/04/16]]
 +
 +
=====Calculations for thiol DNA and ThT solution=====
 +
*AuNP starting concentration: 19.4nM
 +
*DNA starting concentration: 53.1μM
 +
DNA required starting concentration: 5.82μM
 +
(53.1μM)V=(5.82μM)(500μL)
 +
V= 54.8μL 53.1μM thiol DNA in 445.2μL buffer
 +
*thiol DNA concentration required when combined with AuNP: 75×(19.4nM)=1455nM
 +
 +
Combine 250μL 5.82μM thiol DNA and 250μL 5μM ThT
 +
*DNA concentration: 2.91μM
 +
*ThT concentration: 2.5μM
 +
 +
Procedure continued on April 23, 2013
  
  

Revision as of 06:55, 24 April 2013

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April 16, 2013

Reconstituting DNA and Supernatants

10μL of water used to reconstitute each sample of DNA and each of the four supernatants

Gel Electrophoresis

Procedure from Allison Alix 2013/04/16 [[1]]

Calculations for thiol DNA and ThT solution
  • AuNP starting concentration: 19.4nM
  • DNA starting concentration: 53.1μM

DNA required starting concentration: 5.82μM (53.1μM)V=(5.82μM)(500μL) V= 54.8μL 53.1μM thiol DNA in 445.2μL buffer

  • thiol DNA concentration required when combined with AuNP: 75×(19.4nM)=1455nM

Combine 250μL 5.82μM thiol DNA and 250μL 5μM ThT

  • DNA concentration: 2.91μM
  • ThT concentration: 2.5μM

Procedure continued on April 23, 2013