Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/04/09"

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(Entry title)
(April 9, 2013)
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==April 9, 2013==
 
==April 9, 2013==
 
=====Ethanethiol dilutions=====
 
=====Ethanethiol dilutions=====
 +
AuNP: 19.4nM
 +
Ethanethiol: 19.4×15393 = 298624.2nM = 298μM
  
 +
(340mM)V=(30mM)(1mL)
 +
V = 0.088mL = 88μL 340nM in 912μL H<sub>2</sub>O
 +
(30mM)V =(3mM)(1mL)
 +
V = 100μL 30mM in 900μL H<sub>2</sub>O
 +
(3mM)V=(298μM)(1mL)
 +
V = 99μL 3mM in 901μL H<sub>2</sub>O
 +
 +
Combine 200μL 298μM ethanethiol and 200μL 19.4nM AuNP (from March 1, 2013)
 +
=====Absorbance Data=====
 +
[[http://openwetware.org/wiki/User:Allison_K._Alix/Notebook/Thesis_Research/2013/04/09]]
  
  

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April 9, 2013

Ethanethiol dilutions

AuNP: 19.4nM Ethanethiol: 19.4×15393 = 298624.2nM = 298μM

(340mM)V=(30mM)(1mL) V = 0.088mL = 88μL 340nM in 912μL H2O (30mM)V =(3mM)(1mL) V = 100μL 30mM in 900μL H2O (3mM)V=(298μM)(1mL) V = 99μL 3mM in 901μL H2O

Combine 200μL 298μM ethanethiol and 200μL 19.4nM AuNP (from March 1, 2013)

Absorbance Data

[[1]]