Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/03/05"

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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
 
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<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
 
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==Entry title==
+
==March 5, 2013==
* Insert content here...
+
=====Dilution of Molecular Beacon======
 +
*50000pM: (1μM)V=(500000pM)(1mL)
 +
25μL 1μM MB in 475μL buffer
 +
*25000pM: (50000pM)V=(25000)(40μL)
 +
20μL 50000pM in 20μL buffer
 +
*5000pM: (50000pM)V=(5000)(40μL)
 +
4μL 50000pM in 36μL buffer
 +
*500pM: (5000pM)V=(500)(40μL)
 +
4μL 5000pM in 36μL buffer
 +
*100pM: (5000pM)V=(100)(40μL)
 +
1.25μL 50000pM in 38.75μL buffer
 +
*1nm: (50000pM)V=(1000pM)(40μL)
 +
1.25μL 50000pM in 38.75μL buffer
 +
 
 +
=====Sample Data=====
 +
*1:1 1nM <br.>
 +
τ<sub>1</sub>=3.5014 ms <br.>
 +
N=9.2
 +
*1:5 1nM <br.>
 +
τ<sub>1</sub>=0.7290 ms <br.>
 +
N=10
 +
*1:10 1nM <br.>
 +
τ<sub>1</sub>=0.3791 ms <br.>
 +
N=10.9
 +
*5:1 1nM <br.>
 +
τ<sub>1</sub>=0.6540 ms <br.>
 +
N=8
 +
*10:1 1nM <br.>
 +
τ<sub>1</sub>=0.4783 ms <br.>
 +
N=14
 +
*50:1 1nM <br.>
 +
τ<sub>1</sub>=0.4286 ms <br.>
 +
N=3.5
 +
*5:1 500pM <br.>
 +
τ<sub>1</sub>=0.7030 ms <br.>
 +
N=20
 +
*10:1 500pM <br.>
 +
τ<sub>1</sub>=8.0116 ms <br.>
 +
N=7.5
 +
*50:1 500pM <br.>
 +
τ<sub>1</sub>=90515.3 ms <br.>
 +
N=1.91
 +
 
  
  

Latest revision as of 21:33, 26 September 2017

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March 5, 2013

Dilution of Molecular Beacon=
  • 50000pM: (1μM)V=(500000pM)(1mL)

25μL 1μM MB in 475μL buffer

  • 25000pM: (50000pM)V=(25000)(40μL)

20μL 50000pM in 20μL buffer

  • 5000pM: (50000pM)V=(5000)(40μL)

4μL 50000pM in 36μL buffer

  • 500pM: (5000pM)V=(500)(40μL)

4μL 5000pM in 36μL buffer

  • 100pM: (5000pM)V=(100)(40μL)

1.25μL 50000pM in 38.75μL buffer

  • 1nm: (50000pM)V=(1000pM)(40μL)

1.25μL 50000pM in 38.75μL buffer

Sample Data
  • 1:1 1nM <br.>

τ1=3.5014 ms <br.> N=9.2

  • 1:5 1nM <br.>

τ1=0.7290 ms <br.> N=10

  • 1:10 1nM <br.>

τ1=0.3791 ms <br.> N=10.9

  • 5:1 1nM <br.>

τ1=0.6540 ms <br.> N=8

  • 10:1 1nM <br.>

τ1=0.4783 ms <br.> N=14

  • 50:1 1nM <br.>

τ1=0.4286 ms <br.> N=3.5

  • 5:1 500pM <br.>

τ1=0.7030 ms <br.> N=20

  • 10:1 500pM <br.>

τ1=8.0116 ms <br.> N=7.5

  • 50:1 500pM <br.>

τ1=90515.3 ms <br.> N=1.91