Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/02/26"

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(Autocreate 2013/02/26 Entry for User:Madeleine_Y._Bee/Notebook/Single_Molecule_Fluorescence)
 
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==Entry title==
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==February 26, 2013==
* Insert content here...
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* Hybridization of molecular beacon and complementary strand at varying concentrations
 +
===MB:CS (MB at 500pM)===
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*1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS
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(7.3x10^-6M)V=(3x10^-6)(10μL)
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4.1μL MB in 5.9μL buffer
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<br.>
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(2.8x10^-5)V=(3x10^-6M)(10μL)
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1.1μL CS in 8.9μL buffer
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<br.>
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(3x10^-6)V=(500x10^-9)(50μL)
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8.33μL MB in 41.67μL buffer
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*1:5 (1μL MB:5μL CS) - 0.2μM MB:0.8μM CS
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*1:10 (1μL MB:10μL CS) - 0.09μM MB:0.91μM CS
 +
<br.>
 +
===MB:CS (CS at 500pM)===
 +
 
  
  

Revision as of 17:20, 17 March 2013

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February 26, 2013

  • Hybridization of molecular beacon and complementary strand at varying concentrations

MB:CS (MB at 500pM)

  • 1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS

(7.3x10^-6M)V=(3x10^-6)(10μL) 4.1μL MB in 5.9μL buffer <br.> (2.8x10^-5)V=(3x10^-6M)(10μL) 1.1μL CS in 8.9μL buffer <br.> (3x10^-6)V=(500x10^-9)(50μL) 8.33μL MB in 41.67μL buffer

  • 1:5 (1μL MB:5μL CS) - 0.2μM MB:0.8μM CS
  • 1:10 (1μL MB:10μL CS) - 0.09μM MB:0.91μM CS

<br.>

MB:CS (CS at 500pM)