Difference between revisions of "User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/02/26"

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(Entry title)
(February 26, 2013)
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==February 26, 2013==
 
==February 26, 2013==
* Hybridization of molecular beacon and complementary strand at varying concentrations
+
===Hybridization of molecular beacon and complementary strand at varying concentrations===
===MB:CS (MB at 500pM)===
+
====MB:CS (MB at 500pM)====
*1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS
 
 
(7.3x10^-6M)V=(3x10^-6)(10μL)
 
(7.3x10^-6M)V=(3x10^-6)(10μL)
 
4.1μL MB in 5.9μL buffer
 
4.1μL MB in 5.9μL buffer
Line 17: Line 16:
 
<br.>
 
<br.>
 
(3x10^-6)V=(500x10^-9)(50μL)
 
(3x10^-6)V=(500x10^-9)(50μL)
8.33μL MB in 41.67μL buffer
+
8.33μL in 41.67μL  
 +
<br.>
 +
*1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS
 
*1:5 (1μL MB:5μL CS) - 0.2μM MB:0.8μM CS
 
*1:5 (1μL MB:5μL CS) - 0.2μM MB:0.8μM CS
 
*1:10 (1μL MB:10μL CS) - 0.09μM MB:0.91μM CS
 
*1:10 (1μL MB:10μL CS) - 0.09μM MB:0.91μM CS
 
<br.>
 
<br.>
===MB:CS (CS at 500pM)===
+
====MB:CS (CS at 500pM)====
 +
(7.3x10^-6)V=(5x10^-6)(5μL)
 +
3.42μL MB in 1.57μL buffer
 +
<br.>
 +
0.36μL CS in 9.64μL buffer
 +
*1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS
 +
*5:1 (5μL MB:1μL CS) - 0.8μM MB:0.2μM CS
 +
*10:1 (10μL MB:1μL CS) - 0.91μM MB:0.09μM CS
 +
*50:1 (50μL MB:1μL CS) - 0.98μM MB:0.02μM CS
 +
<br.>
 +
Make 1nm, 500pM, and 100pM samples for each ratio
  
  

Revision as of 18:28, 17 March 2013

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February 26, 2013

Hybridization of molecular beacon and complementary strand at varying concentrations

MB:CS (MB at 500pM)

(7.3x10^-6M)V=(3x10^-6)(10μL) 4.1μL MB in 5.9μL buffer <br.> (2.8x10^-5)V=(3x10^-6M)(10μL) 1.1μL CS in 8.9μL buffer <br.> (3x10^-6)V=(500x10^-9)(50μL) 8.33μL in 41.67μL <br.>

  • 1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS
  • 1:5 (1μL MB:5μL CS) - 0.2μM MB:0.8μM CS
  • 1:10 (1μL MB:10μL CS) - 0.09μM MB:0.91μM CS

<br.>

MB:CS (CS at 500pM)

(7.3x10^-6)V=(5x10^-6)(5μL) 3.42μL MB in 1.57μL buffer <br.> 0.36μL CS in 9.64μL buffer

  • 1:1 (1μL MB:1μL CS) - 0.5μM MB:0.5μM CS
  • 5:1 (5μL MB:1μL CS) - 0.8μM MB:0.2μM CS
  • 10:1 (10μL MB:1μL CS) - 0.91μM MB:0.09μM CS
  • 50:1 (50μL MB:1μL CS) - 0.98μM MB:0.02μM CS

<br.> Make 1nm, 500pM, and 100pM samples for each ratio