Difference between revisions of "User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/02/19"

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{|{{table}} width="800"
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Polydopamine Myoglobin Nanofibers</span>
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Polydopamine Myoglobin Nanofibers, Silver Nanofibers</span>
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
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==February 19. 2014==
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==February 19, 2014==
 
===Objectives===
 
===Objectives===
 
*Finish polydopamine nanofiber scaffolds
 
*Finish polydopamine nanofiber scaffolds
===Procedures===
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*Wet-autoclave nanofiber coated scaffold in PBS
*Polydopamine nanofibers
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*Attempt to make silver:protein nanofibers
**Put scaffold into 10mL test tube to conduct Myoglobin nanofiber procedure
 
**Prepare nanofibers according to previously used procedure, with doubled concentration (0.5mM Au in final solution) making 100:1 and 60:1 ratios of gold:myoglobin
 
**Make an additional solution of nanofibers with no scaffold for comparison
 
*Autoclave scaffold in PBS from February 5
 
**Pictures before and after
 
  
===Sample Preparation===
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===Sample Preparation: Polydopamine nanofibers===
 
*Au stock solution:
 
*Au stock solution:
 
**0.011g x (1 mol/393.833g) x 0.010L = 2.793mM
 
**0.011g x (1 mol/393.833g) x 0.010L = 2.793mM
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[[Image:Test1PBS_autoclav_myo10x.jpg|400px]]<br.>
 
[[Image:Test1PBS_autoclav_myo10x.jpg|400px]]<br.>
 
[[Image:Test1PBS_autoclav_myo10x3.jpg|400px]]<br.>
 
[[Image:Test1PBS_autoclav_myo10x3.jpg|400px]]<br.>
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===Sample Preparation: Silver:BSA Nanofiber===
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*We will attempt to form silver:protein nanofibers in a similar method as gold:protein nanofibers, using silver (I) nitrate, BSA, and HCl to bring the pH up to 3 (according to Dr Fox's previous work)
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*The final solution will have 0.25mM AgNO<sub>3</sub>, 1mM HCl, water, and defined ratios of BSA as detailed in the table below
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*All samples will be heated to 80C in an oven for 4 hours
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[[Image:2014_0219_Ag_BSA_nanofibers_ratios.PNG|900px]]
  
  

Revision as of 12:30, 19 February 2014

Owwnotebook icon.png Polydopamine Myoglobin Nanofibers, Silver Nanofibers <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

February 19, 2014

Objectives

  • Finish polydopamine nanofiber scaffolds
  • Wet-autoclave nanofiber coated scaffold in PBS
  • Attempt to make silver:protein nanofibers

Sample Preparation: Polydopamine nanofibers

  • Au stock solution:
    • 0.011g x (1 mol/393.833g) x 0.010L = 2.793mM
  • Myoglobin stock solution:
    • 0.011g x (1 mol/17699g) x 0.010L = 0.0621mM
  • Volumes of Au, protein, and water for each test tube (Myoglobin ratios from Febrary 11):
    • Final [Au]=0.5mM, 5mL total volume
    • 2014 0211 myo bsa NPs.PNG

Autoclaved Nanofiber-coated Scaffold

  • Nanofiber coated scaffold from February 5 wet-autoclaved in PBS
    • Though it appears the nanofibers remained on the scaffold, microscope images show that the scaffold was stained purple and very few nanofibers remained
    • This may be due to autoclaving or to the length of time (14 days) the sample was immersed in PBS

Macro image:<br.> Photo Feb 19, 2 11 50 PM.jpg<br.> Micro images:<br.> Test1PBS autoclav myo10x.jpg<br.> Test1PBS autoclav myo10x3.jpg<br.>

Sample Preparation: Silver:BSA Nanofiber

  • We will attempt to form silver:protein nanofibers in a similar method as gold:protein nanofibers, using silver (I) nitrate, BSA, and HCl to bring the pH up to 3 (according to Dr Fox's previous work)
  • The final solution will have 0.25mM AgNO3, 1mM HCl, water, and defined ratios of BSA as detailed in the table below
  • All samples will be heated to 80C in an oven for 4 hours

2014 0219 Ag BSA nanofibers ratios.PNG