User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/02/19: Difference between revisions

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**Put scaffold into 10mL test tube to conduct Myoglobin nanofiber procedure
**Put scaffold into 10mL test tube to conduct Myoglobin nanofiber procedure
**Prepare nanofibers according to previously used procedure, with doubled concentration (0.5mM Au in final solution) making 100:1 and 60:1 ratios of gold:myoglobin
**Prepare nanofibers according to previously used procedure, with doubled concentration (0.5mM Au in final solution) making 100:1 and 60:1 ratios of gold:myoglobin
**Make an additional solution of nanofibers with no scaffold for comparison
*Autoclave scaffolds in PBS
*Autoclave scaffolds in PBS
**Pictures before and after
**Pictures before and after
===Sample Preparation===
===Sample Preparation===
*Au stock solution:
*Au stock solution:

Revision as of 09:44, 19 February 2014

Polydopamine Myoglobin Nanofibers <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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February 19. 2014

Objectives

  • Finish polydopamine nanofiber scaffolds

Procedures

  • Polydopamine nanofibers
    • Put scaffold into 10mL test tube to conduct Myoglobin nanofiber procedure
    • Prepare nanofibers according to previously used procedure, with doubled concentration (0.5mM Au in final solution) making 100:1 and 60:1 ratios of gold:myoglobin
    • Make an additional solution of nanofibers with no scaffold for comparison
  • Autoclave scaffolds in PBS
    • Pictures before and after

Sample Preparation

  • Au stock solution:
    • 0.011g x (1 mol/393.833g) x 0.010L = 2.793mM
  • Myoglobin stock solution:
    • 0.011g x (1 mol/17699g) x 0.010L = 0.0621mM
  • Volumes of Au, protein, and water for each test tube (Myoglobin ratios from Febrary 11):
    • Final [Au]=0.5mM, 5mL total volume


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