Difference between revisions of "User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/02/12"

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*160:1 BSA
 
*160:1 BSA
 
[[Image:Photo_Feb_19,_2_59_58_PM.jpg|300px]]<br.>
 
[[Image:Photo_Feb_19,_2_59_58_PM.jpg|300px]]<br.>
[[Image:Photo_Feb_19,_3_00_06_PM.jpg|300px]]
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[[Image:Photo_Feb_19,_3_00_06_PM.jpg|400px]]
 
*120:1 BSA
 
*120:1 BSA
 
[[Image:Photo_Feb_19%2C_2_57_38_PM.jpg|300px]]<br.>
 
[[Image:Photo_Feb_19%2C_2_57_38_PM.jpg|300px]]<br.>
[[Image:Photo_Feb_19,_2_57_50_PM.jpg|300px]]
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[[Image:Photo_Feb_19,_2_57_50_PM.jpg|400px]]
 
*80:1 BSA
 
*80:1 BSA
 
[[Image:Photo_Feb_19,_2_58_48_PM.jpg|300px]]<br.>
 
[[Image:Photo_Feb_19,_2_58_48_PM.jpg|300px]]<br.>
[[Image:Photo_Feb_19,_2_58_54_PM.jpg|300px]]
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[[Image:Photo_Feb_19,_2_58_54_PM.jpg|400px]]
 
*60:1 Myoglobin
 
*60:1 Myoglobin
 
[[Image:Photo_Feb_19,_2_56_39_PM.jpg|300px]]<br.>
 
[[Image:Photo_Feb_19,_2_56_39_PM.jpg|300px]]<br.>
[[Image:Photo_Feb_19,_2_56_50_PM.jpg|300px]]
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[[Image:Photo_Feb_19,_2_56_50_PM.jpg|400px]]
 
===Autoclaved Scaffolds===
 
===Autoclaved Scaffolds===
 
*PLA scaffolds were dry autoclaved
 
*PLA scaffolds were dry autoclaved

Revision as of 13:10, 19 February 2014

Owwnotebook icon.png Myoglobin and BSA Nanofibers <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

February 12, 2013

Objectives

  • Pictures of nanofibers from yesterday: macro and micro
  • Run tests on new nanofiber-coated scaffolds (including cell culture, stronger acid, and extended sonication)
  • Brute force method with 0.5mM gold-Myoglobin nanofibers
  • Autoclave one dry, one wet scaffold

Myoglobin and BSA Nanofiber Results

  • 160:1 BSA

Photo Feb 12, 12 17 16 PM.jpg

  • 120:1 BSA

Photo Feb 12, 12 17 31 PM.jpg

  • 80:1 BSA

Photo Feb 12, 12 17 41 PM.jpg

  • 100:1 Myoglobin

Photo Feb 12, 12 17 50 PM.jpg

  • 60:1 Myoglobin

Photo Feb 12, 12 18 06 PM.jpg

Nanofiber Retention Tests

  • For 160:1, 120:1, and 80:1 BSA and 60:1 Myoglobin nanofibers
  1. Sonicate for 30 minutes
  2. Heat at 30C for 30 minutes
  3. Ethanol
  4. PBS
  5. strong acid

Nanofiber Retention Test Results (February 19, 2014)

  • 160:1 BSA

Photo Feb 19, 2 59 58 PM.jpg<br.> <br.> Photo Feb 19, 3 00 06 PM.jpg

  • 120:1 BSA

Photo Feb 19, 2 57 38 PM.jpg<br.> <br.> Photo Feb 19, 2 57 50 PM.jpg

  • 80:1 BSA

Photo Feb 19, 2 58 48 PM.jpg<br.> <br.> Photo Feb 19, 2 58 54 PM.jpg

  • 60:1 Myoglobin

Photo Feb 19, 2 56 39 PM.jpg<br.> <br.> Photo Feb 19, 2 56 50 PM.jpg

Autoclaved Scaffolds

  • PLA scaffolds were dry autoclaved
  • Scaffolds did not melt as expected

Photo Feb 12, 2 49 20 PM.jpg