Difference between revisions of "User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/02/12"

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===Myoglobin and BSA Nanofiber Results===
 
===Myoglobin and BSA Nanofiber Results===
 
*160:1 BSA
 
*160:1 BSA
[[Image:Photo_Feb_12,_12_17_16_PM.jpg]]
+
[[Image:Photo_Feb_12,_12_17_16_PM.jpg|300px]]
 
*120:1 BSA
 
*120:1 BSA
[[Image:Photo_Feb_12,_12_17_31_PM.jpg]]
+
[[Image:Photo_Feb_12,_12_17_31_PM.jpg|300px]]
 
*80:1 BSA
 
*80:1 BSA
[[Image:Photo_Feb_12,_12_17_41_PM.jpg]]
+
[[Image:Photo_Feb_12,_12_17_41_PM.jpg|300px]]
 
*100:1 Myoglobin
 
*100:1 Myoglobin
[[Image:Photo_Feb_12,_12_17_50_PM.jpg]]
+
[[Image:Photo_Feb_12,_12_17_50_PM.jpg|300px]]
 
*60:1 Myoglobin
 
*60:1 Myoglobin
[[Image:Photo_Feb_12,_12_18_06_PM.jpg]]
+
[[Image:Photo_Feb_12,_12_18_06_PM.jpg|300px]]
 
===Nanofiber Retention Tests===
 
===Nanofiber Retention Tests===
 
*For 160:1, 120:1, and 80:1 BSA and 60:1 Myoglobin nanofibers
 
*For 160:1, 120:1, and 80:1 BSA and 60:1 Myoglobin nanofibers

Revision as of 12:48, 19 February 2014

Owwnotebook icon.png Myoglobin and BSA Nanofibers <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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February 12, 2013

Objectives

  • Pictures of nanofibers from yesterday: macro and micro
  • Run tests on new nanofiber-coated scaffolds (including cell culture, stronger acid, and extended sonication)
  • Brute force method with 0.5mM gold-Myoglobin nanofibers
  • Autoclave one dry, one wet scaffold

Myoglobin and BSA Nanofiber Results

  • 160:1 BSA

Photo Feb 12, 12 17 16 PM.jpg

  • 120:1 BSA

Photo Feb 12, 12 17 31 PM.jpg

  • 80:1 BSA

Photo Feb 12, 12 17 41 PM.jpg

  • 100:1 Myoglobin

Photo Feb 12, 12 17 50 PM.jpg

  • 60:1 Myoglobin

Photo Feb 12, 12 18 06 PM.jpg

Nanofiber Retention Tests

  • For 160:1, 120:1, and 80:1 BSA and 60:1 Myoglobin nanofibers
  1. Sonicate for 30 minutes
  2. Heat at 30C for 30 minutes
  3. Ethanol
  4. PBS
  5. strong acid