User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/02/12: Difference between revisions

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===Myoglobin and BSA Nanofiber Results===
===Myoglobin and BSA Nanofiber Results===
*160:1 BSA
*160:1 BSA
 
[[Image:Photo_Feb_12,_12_17_16_PM.jpg]]
*120:1 BSA
*120:1 BSA
 
[[Image:Photo_Feb_12,_12_17_31_PM.jpg]]
*80:1 BSA
*80:1 BSA
 
[[Image:Photo_Feb_12,_12_17_41_PM.jpg]]
*100:1 Myoglobin
*100:1 Myoglobin
 
[[Image:Photo_Feb_12,_12_17_50_PM.jpg]]
*60:1 Myoglobin
*60:1 Myoglobin
[[Image:Photo_Feb_12,_12_18_06_PM.jpg]]
===Nanofiber Retention Tests===
===Nanofiber Retention Tests===
*For 160:1, 120:1, and 80:1 BSA and 60:1 Myoglobin nanofibers
*For 160:1, 120:1, and 80:1 BSA and 60:1 Myoglobin nanofibers

Revision as of 12:47, 19 February 2014

Myoglobin and BSA Nanofibers <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

February 12, 2013

Objectives

  • Pictures of nanofibers from yesterday: macro and micro
  • Run tests on new nanofiber-coated scaffolds (including cell culture, stronger acid, and extended sonication)
  • Brute force method with 0.5mM gold-Myoglobin nanofibers
  • Autoclave one dry, one wet scaffold

Myoglobin and BSA Nanofiber Results

  • 160:1 BSA

  • 120:1 BSA

  • 80:1 BSA

  • 100:1 Myoglobin

  • 60:1 Myoglobin

Nanofiber Retention Tests

  • For 160:1, 120:1, and 80:1 BSA and 60:1 Myoglobin nanofibers
  1. Sonicate for 30 minutes
  2. Heat at 30C for 30 minutes
  3. Ethanol
  4. PBS
  5. strong acid


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