Difference between revisions of "User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/11/12"

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(Autocreate 2013/11/12 Entry for User:Madeleine_Y._Bee/Notebook/CHEM-571_2013F)
 
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==Date==
+
==November 12, 2013==
  
 
===Objective===
 
===Objective===
 +
#Atomic Absorption of nanoparticles
 +
#UV-vis measurements of Lysozyme and Myoglobin nanoparticles
  
 +
===Description===
 +
<u>Atomic Absorption:</u><br.>
 +
*Centrifuge 4mL from each sample for 30min at 4000rpm
 +
*Make standard solutions in the same manner as the last time we performed AA; this time go up to 50ug/mL
 +
*Take measurements of your standards and samples
 +
*Make a graph showing concentration of Au vs Au:protein ratio
 +
=====Gold Standards- Calculations=====
 +
#50 ug/mL:
 +
#*(1000ug/mL)V=(50ug/mL)(5mL)
 +
#*V= 0.25mL Au stock in 4.75mL water
 +
*Each sample diluted from Au stock in the above manner
  
===Description===
 
 
  
 
===Data===
 
===Data===

Revision as of 08:53, 12 November 2013

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November 12, 2013

Objective

  1. Atomic Absorption of nanoparticles
  2. UV-vis measurements of Lysozyme and Myoglobin nanoparticles

Description

Atomic Absorption:<br.>

  • Centrifuge 4mL from each sample for 30min at 4000rpm
  • Make standard solutions in the same manner as the last time we performed AA; this time go up to 50ug/mL
  • Take measurements of your standards and samples
  • Make a graph showing concentration of Au vs Au:protein ratio
Gold Standards- Calculations
  1. 50 ug/mL:
    • (1000ug/mL)V=(50ug/mL)(5mL)
    • V= 0.25mL Au stock in 4.75mL water
  • Each sample diluted from Au stock in the above manner


Data

Notes