User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/11/12: Difference between revisions

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==Date==
==November 12, 2013==


===Objective===
===Objective===
#Atomic Absorption of nanoparticles
#UV-vis measurements of Lysozyme and Myoglobin nanoparticles


===Description===
<u>Atomic Absorption:</u><br.>
*Centrifuge 4mL from each sample for 30min at 4000rpm
*Make standard solutions in the same manner as the last time we performed AA; this time go up to 50ug/mL
*Take measurements of your standards and samples
*Make a graph showing concentration of Au vs Au:protein ratio
=====Gold Standards- Calculations=====
#50 ug/mL:
#*(1000ug/mL)V=(50ug/mL)(5mL)
#*V= 0.25mL Au stock in 4.75mL water
*Each sample diluted from Au stock in the above manner


===Description===


===Data===
===Data===

Revision as of 09:53, 12 November 2013

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November 12, 2013

Objective

  1. Atomic Absorption of nanoparticles
  2. UV-vis measurements of Lysozyme and Myoglobin nanoparticles

Description

Atomic Absorption:<br.>

  • Centrifuge 4mL from each sample for 30min at 4000rpm
  • Make standard solutions in the same manner as the last time we performed AA; this time go up to 50ug/mL
  • Take measurements of your standards and samples
  • Make a graph showing concentration of Au vs Au:protein ratio
Gold Standards- Calculations
  1. 50 ug/mL:
    • (1000ug/mL)V=(50ug/mL)(5mL)
    • V= 0.25mL Au stock in 4.75mL water
  • Each sample diluted from Au stock in the above manner


Data

Notes


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