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(Synthesize Lysozyme AuNPs and Myoglobin AuNPs)
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Revision as of 09:09, 30 October 2013

BDLlogo notext lr.png Experimental Biological Chemistry: Fall 2013 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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October 30, 2013

Objective

Synthesize Lysozyme AuNPs and Myoglobin AuNPs
  • Determine the total amounts of materials you will need for the following samples using the same procedures as the last two times we made protein-AuNPs (final Au concentration of 0.25mM and a final volume of 5mL)
  • Lysozyme
  1. 30
  2. 50
  3. 70
  4. 90
  5. 110
  6. 130
  7. 150
  8. 170
  9. 190
  10. 210
  • Myoglobin
  1. 5
  2. 10
  3. 15
  4. 20
  5. 25
  6. 30
  7. 35
  8. 40
  9. 45
  10. 50

Description

  • Make stock solutions
  • Make samples in small test tubes and cap with aluminum foil
  • Heat samples at 80C for 4 hours

Data

Notes