Difference between revisions of "User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/10/23"

From OpenWetWare
Jump to: navigation, search
(Description)
(Description)
Line 31: Line 31:
 
*Take UV-vis spectrum of each ratio sample
 
*Take UV-vis spectrum of each ratio sample
 
*Save the sample
 
*Save the sample
=====HRP-NP Synthesis=====
+
=====HRP-AuNP Synthesis=====
 
Ratios:
 
Ratios:
 
#230
 
#230
Line 43: Line 43:
 
#390
 
#390
 
#410
 
#410
 +
=====Hemoglobin-AuNPs=====
 +
Ratios:
 +
# 30
 +
# 50
 +
# 70
 +
# 90
 +
# 110
 +
# 130
 +
# 150
 +
# 170
 +
# 190
 +
# 210
  
 
===Data===
 
===Data===

Revision as of 08:23, 23 October 2013

BDLlogo notext lr.png Experimental Biological Chemistry: Fall 2013 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


October 23, 2013

Happy Mole Day!

Objective

To measure the activity of pepsin in solution and pepsin-AuNPs in solution.

Description

Pepsin-AuNP Analysis

(picture)<br.> Physical Characteristics (by ratio):<br.>

  1. 30-
  2. 50-
  3. 70-
  4. 90-
  5. 110-
  6. 130-
  7. 150-
  8. 170-
  9. 190-
  10. 210-

<br.>

  • Centrifuge 1mL of each ratio of NPs for 30 minutes
  • Take UV-vis spectrum of each ratio sample
  • Save the sample
HRP-AuNP Synthesis

Ratios:

  1. 230
  2. 250
  3. 270
  4. 290
  5. 310
  6. 330
  7. 350
  8. 370
  9. 390
  10. 410
Hemoglobin-AuNPs

Ratios:

  1. 30
  2. 50
  3. 70
  4. 90
  5. 110
  6. 130
  7. 150
  8. 170
  9. 190
  10. 210

Data

Notes