Difference between revisions of "User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/10/15"

From OpenWetWare
Jump to: navigation, search
Line 19: Line 19:
 
*90:1 - 0.25mM:2.78uM - 850uL:1.71mL, diluted up to 10mL in a volumetric flask
 
*90:1 - 0.25mM:2.78uM - 850uL:1.71mL, diluted up to 10mL in a volumetric flask
 
*120:1 - 0.25mM:2.08uM - 850uL:1.28mL, diluted up to 10mL in a volumetric flask
 
*120:1 - 0.25mM:2.08uM - 850uL:1.28mL, diluted up to 10mL in a volumetric flask
 +
Leave samples in 80C oven for three hours.
  
===Data===
 
 
 
===Notes===
 
  
  

Revision as of 08:40, 15 October 2013

BDLlogo notext lr.png Experimental Biological Chemistry: Fall 2013 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


October 15, 2013

Objective

Make four samples of HRP-AuNPs.

Description

Au:HRP Ratios (with corresponding concentrations and volumes to make 10mL)<br.>

  • 30:1 - 0.25mM:8.33uM - 850uL:5.11mL, diluted up to 10mL in a volumetric flask
  • 60:1 - 0.25mM:4.17uM - 850uL:2.56mL, diluted up to 10mL in a volumetric flask
  • 90:1 - 0.25mM:2.78uM - 850uL:1.71mL, diluted up to 10mL in a volumetric flask
  • 120:1 - 0.25mM:2.08uM - 850uL:1.28mL, diluted up to 10mL in a volumetric flask

Leave samples in 80C oven for three hours.