Difference between revisions of "User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/10/01"

From OpenWetWare
Line 24: Line 24:
====Absorbance Measurements====
<u>Absorbance Measurements</u><br.>
<u>Beer's Law concentration calculations</u><br.>

Revision as of 08:49, 2 October 2013

BDLlogo notext lr.png Experimental Biological Chemistry: Fall 2013 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

October 1, 2013


  1. UV-Vis Absorbance of reactants, catalysts, and products
    • Make 10mL stock solution of luminol at appropriate dilution
  2. Kinetics of luminol oxidized by changes in absorption spectrum, reaction carried out in stopped flow mixer


UV-vis Samples<br.>

  • Blank: TRIS buffer
  • Horseradish peroxidase
  • H2O2
  • 20x luminol
  • 10x 3-aminophthalic acid (product of reaction between 333uL luminol, 333uL H2O2 catalyzed by 333uL HRP)


Absorbance Measurements<br.> 2013 1001 absorbance.PNG<br.> Beer's Law concentration calculations<br.> 2013 1001 beer's law calcs.PNG<br.>