Difference between revisions of "User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/10/01"

From OpenWetWare
Jump to: navigation, search
(Autocreate 2013/10/01 Entry for User:Madeleine_Y._Bee/Notebook/CHEM-571_2013F)
 
 
(2 intermediate revisions by the same user not shown)
Line 8: Line 8:
  
  
==Date==
+
==October 1, 2013==
  
 
===Objective===
 
===Objective===
 
+
#UV-Vis Absorbance of reactants, catalysts, and products
 +
#*Make 10mL stock solution of luminol at appropriate dilution
 +
#Kinetics of luminol oxidized by changes in absorption spectrum, reaction carried out in stopped flow mixer
  
 
===Description===
 
===Description===
#
+
<u>UV-vis Samples</u><br.>
 +
*Blank: TRIS buffer
 +
*Horseradish peroxidase
 +
*H<sub>2</sub>O<sub>2</sub>
 +
*20x luminol
 +
*10x 3-aminophthalic acid (product of reaction between 333uL luminol, 333uL H<sub>2</sub>O<sub>2</sub> catalyzed by 333uL HRP)
  
 
===Data===
 
===Data===
*
+
<u>Absorbance Measurements</u><br.>
 +
[[Image:2013_1001_absorbance.PNG]]<br.>
 +
<u>Beer's Law concentration calculations</u><br.>
 +
[[Image:2013_1001_beer's_law_calcs.PNG]]<br.>
  
===Notes===
+
<u>Kinetic Measurements</u>
  
  
 +
===Notes===
  
[[Category:]]
 
[[Category:Miscellaneous]]
 
  
  

Latest revision as of 09:13, 2 October 2013

BDLlogo notext lr.png Experimental Biological Chemistry: Fall 2013 <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>      </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


October 1, 2013

Objective

  1. UV-Vis Absorbance of reactants, catalysts, and products
    • Make 10mL stock solution of luminol at appropriate dilution
  2. Kinetics of luminol oxidized by changes in absorption spectrum, reaction carried out in stopped flow mixer

Description

UV-vis Samples<br.>

  • Blank: TRIS buffer
  • Horseradish peroxidase
  • H2O2
  • 20x luminol
  • 10x 3-aminophthalic acid (product of reaction between 333uL luminol, 333uL H2O2 catalyzed by 333uL HRP)

Data

Absorbance Measurements<br.> 2013 1001 absorbance.PNG<br.> Beer's Law concentration calculations<br.> 2013 1001 beer's law calcs.PNG<br.>

Kinetic Measurements


Notes