Difference between revisions of "User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/09/04"

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==September 4,2013==
 
==September 4,2013==
 
===Objective===
 
===Objective===
Execute the following [http://openwetware.org/wiki/User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/04 procedure] examining protein fluoresecence.
+
Continue procedure from [http://openwetware.org/wiki/User:Madeleine_Y._Bee/Notebook/CHEM-571_2013F/2013/09/03 yesterday].
===Procedure===
 
*Selected protein:
 
 
 
  
 
===Data===
 
===Data===
====Absorbance Measurements====
+
====Absorption Spectra====
 +
[[Image:2013_0904_inosine_spectra.PNG]]<br.>
 +
All peaks at 249nm. <br.>
  
====Fluorescence Measurements====
+
===Calculations and Analysis===
 +
====Calibration Curves====
 +
[[Image:2013_0904_inosine_calibration.PNG]]
  
===Analysis===
+
=====Determining Unknown's Concentration=====
====Integration====
+
*Unknown from:
 +
*Concentration determined from calibration curve:
 +
*Actual concentration:
  
====Normalization====
 
  
  

Revision as of 09:22, 4 September 2013

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September 4,2013

Objective

Continue procedure from yesterday.

Data

Absorption Spectra

2013 0904 inosine spectra.PNG<br.> All peaks at 249nm. <br.>

Calculations and Analysis

Calibration Curves

2013 0904 inosine calibration.PNG

Determining Unknown's Concentration
  • Unknown from:
  • Concentration determined from calibration curve:
  • Actual concentration: