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(Determining Unknown's Concentration)
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*Concentration determined from calibration curve:
 
*Concentration determined from calibration curve:
 
*Actual concentration:
 
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Revision as of 12:27, 3 September 2013

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September 3, 2013

Objective

Execute Dr Hartings' procedure by making several dilutions of adenosine and inosine solutions, measuring adsorption spectra, constructing calibration curves, performing statistical analyses, and determining concentration of 'unknowns' using calibration curves.

Procedure Prep

Stock Solutions and Dilutions

Adenosine MW: 267.24g/mol<br.> Inosine MW: 268.2g/mol<br.> <br.> Original stock solutions:<br.> Adenosine: (from 0.1091g in 50mL volumetric flask) 0.0082M<br.> Inosine: (from 0.1060g in 50mL volumetric flask) 0.0079M<br.> <br.> Dilutions to make, each with total volumes of 1mL:<br.>

Adenosine solution concentrations (M) Volume stock solution added (L) Inosine solution concentrations (M) Volume of stock solution added (L)
3.00x10-5 3.66x10-6 4.80x10-5 6.08x10-6
2.50x10-5 3.05x10-6 4.00x10-5 5.06x10-6
2.00x10-5 2.44x10-6 3.20x10-5 4.05x10-6
1.50x10-5 1.83x10-6 2.40x10-5 3.04x10-6
1.00x10-5 1.22x10-6 1.60x10-5 2.03x10-6
0.50x10-5 6.10x10-7 0.80x10-5 1.01x10-6
RANDOM: 0.25x10-5 3.05x10-7 RANDOM: 0.40x10-5 5.06x10-7

Data

Absorption Spectra

2013 0903 adenosine spectra.PNG<br.> All peaks at 259nm.

Calculations and Analysis

Determine the standard deviation for your data points.<br.> Determine the confidence interval for 90% and 95% confidence.<br.> Determine if any data can be ruled out using a Q-test.<br.>

Calibration Curves

2013 0903 adenosine calibration.PNG

Determining Unknown's Concentration

  • Unknown from:
  • Concentration determined from calibration curve:
  • Actual concentration: