Difference between revisions of "User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/09/03"

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(Stock Solutions and Dilutions)
(Calibration Curves)
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Determine if any data can be ruled out using a Q-test.<br.>
Determine if any data can be ruled out using a Q-test.<br.>
====Calibration Curves====
====Calibration Curves====
====Determining Unknown's Concentration====
====Determining Unknown's Concentration====

Revision as of 11:12, 3 September 2013

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September 3, 2013


Execute Dr Hartings' procedureby making several dilutions of adenosine and inosine solutions, measuring adsorption spectra, constructing calibration curves, and determining concentration of 'unknowns' using calibration curves.

Procedure Prep

Stock Solutions and Dilutions

Adenosine MW:267.24g/mol<br.> Inosine MW:268.2g/mol<br.> <br.> Original stock solutions:<br.> Adenosine: (from 0.1091g in 50mL volumetric flask) 0.0082M<br.> Inosine: (from 0.1060g in 50mL volumetric flask) 0.0079M<br.> <br.> Dilutions to make, each with total volumes of 1mL:<br.>

Adenosine solution concentrations (M) Volume stock solution added (L) Inosine solution concentrations (M) Volume of stock solution added (L)
3.00x10-5 3.66x10-6 4.80x10-5 6.08x10-6
2.50x10-5 3.05x10-6 4.00x10-5 5.06x10-6
2.00x10-5 2.44x10-6 3.20x10-5 4.05x10-6
1.50x10-5 1.83x10-6 2.40x10-5 3.04x10-6
1.00x10-5 1.22x10-6 1.60x10-5 2.03x10-6
0.50x10-5 6.10x10-7 0.80x10-5 1.01x10-6
RANDOM: 0.25x10-5 3.05x10-7 RANDOM: 0.40x10-5 5.06x10-7


Absorption Spectra

2013 0903 adenosine spectra.PNG

Calculations and Analysis

Determine the standard deviation for your data points.<br.> Determine the confidence interval for 90% and 95% confidence.<br.> Determine if any data can be ruled out using a Q-test.<br.>

Calibration Curves

2013 0903 adenosine calibration.PNG

Determining Unknown's Concentration