User:Lu Wang/Notebook/Team Allergy/2010/07/28: Difference between revisions
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== | ==Protocol== | ||
Yesterday, we plated E. coli containing complete hpRNA for Ger, Bet v2, and LTP. Bet v1 did not ligate properly in the earlier Sense+Intron ligation. | |||
Today, we will extract the plasmids from the E. coli and prepare them for sequencing tomorrow. We will also do ligations with the false negatives (Sense + PDK ligate with Antisense). | |||
#Culture colonies | |||
#Miniprep | |||
#Nanodrop | |||
#Digest false negatives | |||
#Ligate | |||
#Transform | |||
==Results== | |||
'''Nanodrop''' | |||
The format is: Miniprep # , Name of Allergen, concentration in ng/μL of colony 1, concentration in ng/μL of colony 2. | |||
All these working ligations are with the PAL intron | |||
#9, LTP, 97.9, 94.1 | |||
#11, LTP, 77.9, 94.8 | |||
#25, Bet v2, 67.8, 97.8 (there was a mixup with colony 1 and LTP+PDK, but it is now fixed in our electronic notebooks) | |||
#28, Bet v2, 73.2, 83.6 | |||
#36, Ger3, 76.1, 83.4 | |||
#37, Ger3, 70.0, 75.3 | |||
#38, Ger3, 90.4, 108.3 | |||
*Transformed more sense+pdk parts for our false negatives (16,30,31) | |||
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Revision as of 12:00, 30 July 2010
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ProtocolYesterday, we plated E. coli containing complete hpRNA for Ger, Bet v2, and LTP. Bet v1 did not ligate properly in the earlier Sense+Intron ligation. Today, we will extract the plasmids from the E. coli and prepare them for sequencing tomorrow. We will also do ligations with the false negatives (Sense + PDK ligate with Antisense).
ResultsNanodrop The format is: Miniprep # , Name of Allergen, concentration in ng/μL of colony 1, concentration in ng/μL of colony 2. All these working ligations are with the PAL intron
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